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Journal: Frontiers in Pharmacology
Article Title: Aqueous extracts of Portulaca oleracea L. alleviate atopic dermatitis by restoring skin barrier function
doi: 10.3389/fphar.2025.1591394
Figure Lengend Snippet: PO carbomer hydrogel modulates cutaneous immune responses and suppresses the generation of proinflammatory factors in DNCB-induced AD mice. (A) Immunohistochemical staining of CD4 + cell infiltration (brown) in the skin. The scale bar represents 100 μm. (B) Quantification of the CD4 + area in the skin. (C) Relative CD4 protein expression in dorsal skin. (D) Relative mRNA expression of Ifn-γ , Il4 , FcεRIα, and Il17a in dorsal skin, as detected by quantitative PCR. n = 6. (E,F) Determination of IgE, INF-γ, IL-4, and IL-17 concentrations in mouse serum (E) and affected skin (F) via ELISA. The data are expressed as means ± SD. # p < 0.05, ## p < 0.01, ### p < 0.001 vs. the vehicle control (Ctr) group; * p < 0.05, ** p < 0.01, and *** p < 0.001 vs. the DNCB-induced AD group.
Article Snippet: After permeabilization, the sections were incubated overnight at 4°C with a
Techniques: Immunohistochemical staining, Staining, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Control
Journal: Frontiers in Immunology
Article Title: Alcohol intake exacerbates experimental autoimmune prostatitis through activating PI3K/AKT/mTOR pathway-mediated Th1 differentiation
doi: 10.3389/fimmu.2024.1512456
Figure Lengend Snippet: In vitro experiments further demonstrated increased proportion of Th1 cells and the pathway activation. (A) Representative pictures of flow cytometric staining for Th1 cells of Naive CD4+, Th1, Th1-alcohol, Th1-alcohol-shPI3K group. (B) Flow cytometric analysis of the proportion of Th1 cells in Naive CD4+, Th1, Th1-alcohol, Th1-alcohol-shPI3K group. (C) Representative pictures of immunofluorescent staining for Th1 cells of Naive CD4+, Th1, Th1-alcohol, Th1-alcohol-shPI3K. (D) The expressions of AKT, phospho-AKT, mTOR, and phospho-mTOR in lymphocytes were detected by Western blotting analysis for the Naive CD4+, Th1, Th1-alcohol, Th1-alcohol-shPI3K group. (E) Relative density analysis of AKT/phospho-AKT. (F) Relative density analysis of mTOR/phospho-mTOR. NS, no significance, ***P < 0.001, ****P < 0.0001; EAP, experimental autoimmune prostatitis; EV, EAP-vehicle, E-LY, EAP-LY294002, EA, EAP-alcohol, EA-LY, EAP-alcohol-LY294002.
Article Snippet: The
Techniques: In Vitro, Activation Assay, Staining, Western Blot